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JoVE Journal Biology
Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells
 

Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells

Article doi: 10.3791/58112
September 26th, 2018 •
*1, *1, 2, 2, 1, 1
1Enteric Neuroscience Program, Division of Gastroenterology & Hepatology,Department of Physiology & Biomedical Engineering, Mayo Clinic, 2Division of Gastroenterology, Department of Medicine, University of Massachusetts Medical School
* These authors contributed equally

Summary
Automatic Translation
September 26th, 2018

Enterochromaffin (EC) cells comprise a small subset of gastrointestinal epithelial cells. EC cells are electrically excitable and release serotonin, yet difficulties in culturing and identifying EC cells have limited physiological studies. The method presented here establishes a primary culture model amenable to examination of single EC cells by electrophysiology.

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