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Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells
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JoVE Journal Biology
Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells

Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells

DOI:
10.3791/58112-v

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10:04 min

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September 26, 2018

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, , , , ,
1Enteric Neuroscience Program, Division of Gastroenterology & Hepatology,Department of Physiology & Biomedical Engineering,Mayo Clinic, 2Division of Gastroenterology, Department of Medicine,University of Massachusetts Medical School

Chapters

  • 00:04Title
  • 00:45Tissue Isolation
  • 02:08Enzymatic and Mechanical Digestion
  • 04:20Cell Culture
  • 05:16Whole Cell Electrophysiology of EC Cells from Primary Culture
  • 08:06Results: Culture Results and Throughput of Whole-cell Electrophysiology
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

Enterochromaffin (EC) cells comprise a small subset of gastrointestinal epithelial cells. EC cells are electrically excitable and release serotonin, yet difficulties in culturing and identifying EC cells have limited physiological studies. The method presented here establishes a primary culture model amenable to examination of single EC cells by electrophysiology.

Tags

Keywords: Whole Cell ElectrophysiologyPrimary Cultured Murine Enterochromaffin CellsEnteric NeuroscienceGastrointestinal PhysiologyEnterochromaffin Cell ExcitabilityGastrointestinal Hormone ReleaseSingle-cell TechniquesElectrophysiologyPBSMicrodissectionCollagenase Digestion

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