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Visualisering af endoplasmatiske reticulum Subdomains i dyrkede celler
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Visualisering af endoplasmatiske reticulum Subdomains i dyrkede celler

Article DOI: 10.3791/50985-v • 16:43 min • February 18th, 2014
February 18th, 2014 •
1,2,3, 2,3,4, 2,3, 1,2
1Fondazione Filarete, 2Department of Biotechnology and Translational Medicine, University of Milan, 3Neuroscience Institute, National Research Council (CNR), 4Department of Health Science, "Magna Graecia" University of Catanzaro

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Vi beskriver de billeddannende metoder, vi bruger til at undersøge fordelingen og mobilitet for de transficerede fluorescerende proteiner er hjemmehørende i det endoplasmatiske reticulum (ER) ved hjælp af konfokal billedbehandling af levende celler. Vi har også ultrastrukturelt analysere effekten af ​​deres udtryk på arkitektur i dette subcellulære rum.

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Mikrobiologi endoplasmatiske reticulum (ER) fluorescerende proteiner (fps) konfokal mikroskopi fluorescens bedring efter fotoblegning (FRAP) fluorescens tab fotoblegning (FLIP) ultrastruktur transmission elektron mikroskopi (TEM)
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