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Hele cellen Elektrofysiologi primær kulturperler Murine Enterochromaffin celler
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JoVE Journal Biology
Whole Cell Electrophysiology of Primary Cultured Murine Enterochromaffin Cells

Hele cellen Elektrofysiologi primær kulturperler Murine Enterochromaffin celler

DOI:
10.3791/58112-v

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10:04 min

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September 26, 2018

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, , , , ,
1Enteric Neuroscience Program, Division of Gastroenterology & Hepatology,Department of Physiology & Biomedical Engineering,Mayo Clinic, 2Division of Gastroenterology, Department of Medicine,University of Massachusetts Medical School

Chapters

  • 00:04Title
  • 00:45Tissue Isolation
  • 02:08Enzymatic and Mechanical Digestion
  • 04:20Cell Culture
  • 05:16Whole Cell Electrophysiology of EC Cells from Primary Culture
  • 08:06Results: Culture Results and Throughput of Whole-cell Electrophysiology
  • 09:18Conclusion

Summary

Automatic Translation

Automatic Translation

Enterochromaffin (EF) celler omfatter en lille delmængde af gastrointestinale epitelceller. EF celler er elektrisk overgearet og frigive serotonin, men vanskeligheder i dyrkning og identificere EF celler har begrænset fysiologiske undersøgelser. Metoden præsenteres her etablerer en primær kultur model imødekommenhed over for undersøgelse af enkelt EF celler af Elektrofysiologi.

Tags

Whole Cell ElectrophysiologyPrimary Cultured Murine Enterochromaffin CellsEnteric NeuroscienceGastrointestinal PhysiologyEnterochromaffin Cell ExcitabilityGastrointestinal Hormone ReleaseSingle-cell TechniquesElectrophysiologyPBSMicrodissectionCollagenase Digestion

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