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Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis
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Neuroscience
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JoVE Journal Neuroscience
Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis

Dissection of the Transversus Abdominis Muscle for Whole-mount Neuromuscular Junction Analysis

DOI:
10.3791/51162-v

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06:12 min

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January 11, 2014

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, ,
1Regenerative Medicine Program,Ottawa Hospital Research Institute, 2Centre for Integrative Physiology,University of Edinburgh

Chapters

  • 00:00Title
  • 00:34Dissection of Abdominal Musculature from a Recently Euthanized Mouse
  • 02:15Isolation of the TVA Muscle
  • 04:17Immunofluorescent Labeling and Microscopy of the TVA Muscle
  • 05:04Conclusions and Results

Summary

Automatic Translation

Automatic Translation

In this video we demonstrate a protocol for dissection of the transversus abdominis muscle of the mouse and use immunofluorescence and microscopy to visualize neuromuscular junctions.

Tags

Keywords: Transversus AbdominisNeuromuscular JunctionMotor NeuronDissectionImmunofluorescenceSpinal Muscular AtrophyMotor UnitAxonal BranchingTerminal SproutingInnervation Pattern

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