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Analysere proteasomal Nedsatt en Cell-free systemet i planter
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Analysere proteasomal Nedsatt en Cell-free systemet i planter

Article DOI: 10.3791/51293-v • 07:43 min • March 26th, 2014
March 26th, 2014 •
1, 1, 1
1Department of Biochemistry and Cell Biology, Stony Brook University, State University of New York

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Målrettet protein degradering representerer en viktig reguleringsmekanisme for cellefunksjon. Det skjer via en konservert Ubiquitin-proteasom, som festes polyubiquitin kjedene til målet protein som deretter tjene som molekylære "koder" for 26S proteasomet. Her beskrives en enkel og pålitelig cellefritt assay for proteasomal degradering av proteiner.

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Biokjemi Ubiquitin / proteasome system 26S proteasome protein degradering proteasominhibitor Western blotting plantegenetisk transformasjon
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