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Journal / Immunology and Infection / Isolering af humanmonocytter af Double Gradient…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering af humanmonocytter af Double Gradient centrifugering og deres differentiering til makrofager i teflonbelagte Cell Culture Tasker
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Isolering af humanmonocytter af Double Gradient centrifugering og deres differentiering til makrofager i teflonbelagte Cell Culture Tasker

Article DOI: 10.3791/51554-v • 09:32 min • September 9th, 2014
September 9th, 2014 •
1, 1, 1, 2, 1, 1, 1
1Department of Hematology and Oncology, University Medical Center Göttingen, 2Microbial Interface Biology Group, Research Center Borstel

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Vi præsenterer en enkel og effektiv protokol til generering af humane makrofager. Buffy coats behandles af dobbelt densitetsgradientcentrifugering og isolerede monocytter derefter differentieres til makrofager i teflonbelagte cellekultur poser. Dette maksimerer makrofag udbytter og letter cellehøst til efterfølgende eksperimenter.

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Immunologi makrofager monocytter isoleret PBMC'er densitetsgradient differentiering teflonbelagte cellekultur poser
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