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siRNA Screening for å identifisere Ubiquitin og Ubiquitin-lignende system regulatorer av biologiske mekanismer i dyrkede pattedyrceller
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siRNA Screening for å identifisere Ubiquitin og Ubiquitin-lignende system regulatorer av biologiske mekanismer i dyrkede pattedyrceller

Article DOI: 10.3791/51572-v • 10:43 min • May 24th, 2014
May 24th, 2014 •
1, 1, 1, 2, 1,2
1MRC Protein Phosphorylation and Ubiquitylation Unit, College of Life Sciences, University of Dundee, 2Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee

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Her beskriver vi en metode for å utføre en målrettet siRNA "ubiquitome" skjermen for å identifisere romanen ubiquitin og ubiquitin-lignende regulatorer av HIF1A-mediert cellulær respons på hypoksi. Dette kan tilpasses til en hvilken som helst biologisk vei, hvor et robust lest ut av reporter-aktivitet er tilgjengelig.

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Biokjemi siRNA screening ubiquitin UBL ubiquitome hypoksi HIF1A High-throughput pattedyrceller luciferase reporter
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