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In vivo mikroinjektion och elektroporation av Mus Testikel

Article DOI: 10.3791/51802-v • 08:39 min • August 23rd, 2014
August 23rd, 2014 •
1, 1, 1
1Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)

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I denna artikel beskrivs mikroinjektion och elektroporering av mus testiklar in vivo som en transfektion teknik för testikel musceller att studera unika processer av spermatogenesen. Den presenterade protokollet involverar stegen att preparatet glaskapillär, mikroinjektion via efferenta kanalen, och transfektering genom elektroporation.

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Molecular Biology elektroporering transfektion mikroinjektion testikel spermier spermatogenes reproduktion
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