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JoVE Journal
Bioengineering

This content is Open Access.

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles
 

Analysis of Tubular Membrane Networks in Cardiac Myocytes from Atria and Ventricles

Article doi: 10.3791/51823
October 15th, 2014

Summary October 15th, 2014

In cardiac myocytes, tubular membrane structures form intracellular networks. We describe optimized protocols for i) isolation of myocytes from mouse heart including quality control, ii) live cell staining for state-of-the-art fluorescence microscopy, and iii) direct image analysis to quantify the component complexity and the plasticity of intracellular membrane networks.

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