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JoVE Journal
Bioengineering

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Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting
 

Protocol for MicroRNA Transfer into Adult Bone Marrow-derived Hematopoietic Stem Cells to Enable Cell Engineering Combined with Magnetic Targeting

Article DOI: 10.3791/57474 11:37 min June 18th, 2018
June 18th, 2018

Chapters

Summary

This protocol illustrates a safe and efficient procedure to modify CD133+ hematopoietic stem cells. The presented non-viral, magnetic polyplex-based approach may provide a basis for the optimization of therapeutic stem cell effects as well as for monitoring the administered cell product via magnetic resonance imaging.

Transcript

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MicroRNA Transfer Adult Bone Marrow-derived Hematopoietic Stem Cells Cell Engineering Magnetic Targeting Regenerative Medicine Non-viral Transfection Method Sauter's Method Modification Of Hematopoietic Stem Cells Mesenchymal Stem Cells Protocol Human Lymphocyte Medium Collagenase B DNase Flow Cytometric Analysis PBS EDTA Density Gradient Centrifugation Tube
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