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JoVE Journal Biology
Erzeugung
Accelerating Research on Bacterial Extracellular Vesicles Separation and Heterogeneity (Video) | JoVE
Journal (Biology)
Accelerating Research on Bacterial Extracellular Vesicles Separation and Heterogeneity (Video) | JoVE
Light/dark Transition Test for Mice
Journal (Biology)
Light/dark Transition Test for Mice
 
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Erzeugung

Article DOI: 10.3791/51934-v • 13:31 min • October 31st, 2014
October 31st, 2014 •
1
1Department of Biological Sciences, Center for Applied Chemical Biology, Youngstown State University

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Enterobacter sp. Ysu wächst in Glucose-Minimalsalzmedium. Auxotrophe werden durch Transformation mit einem transposome die zufällig fügt sich in das Wirtsgenom erzeugt. Mutanten werden durch Replika-Plattierung aus komplexen Medium, um Minimalmedium gefunden. Unterbrochen Gene durch Gen Rettungs- und Sequenzierung identifiziert.

Tags

Mikrobiologie Auxotrophie transposome Transposons Mutagenese Replikaplattierung Glucose-Minimalmedium komplexes Medium,
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