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的产生<em>肠杆菌</em> SP。 YSU营养缺陷型使用转座子突变
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Biology
Generation of
Enterobacter
sp. YSU Auxotrophs Using Transposon Mutagenesis
Please note that all translations are automatically generated.
Click here for the English version.
的产生<em>肠杆菌</em> SP。 YSU营养缺陷型使用转座子突变
DOI:
10.3791/51934-v
•
13:31 min
•
October 31, 2014
•
Jonathan James Caguiat
1
Department of Biological Sciences, Center for Applied Chemical Biology
,
Youngstown State University
Chapters
00:05
Title
02:27
Electroporation of Competent Cells
04:35
Gridding Transformants and Replica Plating to Determine the Auxotroph Phenotype
08:02
Gene Rescue
11:13
Results: Generation of Auxotrophic Transformants in Enterobacter sp. YSU
12:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
肠杆菌
属。 YSU生长在最小的葡萄糖盐培养基。通过用转座子其中随机插入本身入宿主基因组转化它产生营养缺陷型。突变体是通过影印从复合培养基到基本培养基中。打断了基因鉴定的基因救援和测序。
Tags
Enterobacter Sp. YSU
Auxotrophs
Transposon Mutagenesis
Tn5 Transposome
M-9 Minimal Medium
R6K Gamma Replication Origin
Kanamycin Resistance
Sanger Sequencing
BLAST
Gene Identification
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