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October 31, 2014
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肠杆菌属。 YSU生长在最小的葡萄糖盐培养基。通过用转座子其中随机插入本身入宿主基因组转化它产生营养缺陷型。突变体是通过影印从复合培养基到基本培养基中。打断了基因鉴定的基因救援和测序。
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Cite this Article
Caguiat, J. J. Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis. J. Vis. Exp. (92), e51934, doi:10.3791/51934 (2014).
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