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An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

DOI:
10.3791/51948-v

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10:36 min

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September 24, 2014

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, , ,
1Department of Mechanical Engineering,San José State University, 2J. Crayton Pruitt Family Department of Biomedical Engineering,University of Florida, 3Department of Physiology,Emory University School of Medicine, 4Department of Biological Sciences,San José State University

Chapters

  • 00:05Title
  • 01:28Dissection of EDL Muscle and Nerve
  • 04:42Mounting the EDL Muscle and Nerve in the Tissue Bath
  • 07:44Data Collection
  • 08:51Results: Spindle Afferent Response to Twitch Contraction and Ramp-and-Hold Stretch
  • 10:02Conclusion

Summary

Automatic Translation

Automatic Translation

Muscle sensory neurons are involved in proprioceptor signaling and also report on metabolic state and injury related events. We describe an adult mouse in vitro muscle-nerve preparation for studies on stretch-activated muscle afferents.

Tags

Keywords: Muscle AfferentsMuscle SpindleGolgi Tendon OrganProprioceptionMuscle Sensory NeuronsExtensor Digitorum LongusIn Vitro PreparationStretch-evoked ResponseSpike SortingMuscle PerfusionGenetic ApproachesDisease Models

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