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An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents
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An In Vitro Adult Mouse Muscle-nerve Preparation for Studying the Firing Properties of Muscle Afferents

Article DOI: 10.3791/51948 • 10:36 min • September 24th, 2014
September 24th, 2014 •
1, 2, 3, 4
1Department of Mechanical Engineering, San José State University, 2J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 3Department of Physiology, Emory University School of Medicine, 4Department of Biological Sciences, San José State University

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Summary
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Muscle sensory neurons are involved in proprioceptor signaling and also report on metabolic state and injury related events. We describe an adult mouse in vitro muscle-nerve preparation for studies on stretch-activated muscle afferents.

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In Vitro Adult Mouse Muscle-nerve Preparation Muscle Afferents Firing Properties Muscle Sensory Neurons Muscle Spindles Golgi Tendon Organs Proprioception Afferent Fibers Metabolite Buildup Temperature Nociceptive Stimuli Abnormal Activation Movement Disorders Chronic Pain Syndromes Extensor Digitorum Longus (EDL) Muscle Stretch-evoked Afferent Responses Suction Electrode Spike Sorting Software Anesthesia Altered Muscle Perfusion Protocol Muscle Spindle Afferents
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