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High Efficiency Differenzierung von humanen pluripotenten Stammzellen zu Kardiomyozyten und Charakterisierung mittels Durchflusszytometrie
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High Efficiency Differenzierung von humanen pluripotenten Stammzellen zu Kardiomyozyten und Charakterisierung mittels Durchflusszytometrie

Article DOI: 10.3791/52010-v • 13:13 min • September 23rd, 2014
September 23rd, 2014 •
*1, *2, 1, 1, 3, 2, 4,5, 1,6
1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin
* These authors contributed equally

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Der Artikel beschreibt die detaillierte Methode zur menschlichen pluripotenten Stammzellen differenzieren zu Herzmuskelzellen wirksam durch selektives Modulieren des Wnt-Signalwegs, gefolgt von Durchflusszytometrie-Analyse von Referenzmarkierungen, um die Homogenität und Identität der Population abzuschätzen.

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Cellular Biology Ausgabe 91 menschlichen induzierten pluripotenten Stammzellen Durchflusszytometrie gerichtet Differenzierung Kardiomyozyten IRX4 TNNI3 TNNT2 MCL2v MLC2a
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