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1Department of Biochemistry,Medical College of Wisconsin, 2Stanford Cardiovascular Institute,Stanford University School of Medicine, 3Department of Anesthesiology,Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine,Hong Kong University, 5Division of Cardiology,Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center,Medical College of Wisconsin
Chapters
- 00:05Title
- 01:43Passaging and Maintenance of Undifferentiated hPSCs in Monolayer Culture
- 05:18Cardiomyocyte Induction of hPSCs by Selective Modulation of the Wnt Pathway
- 08:22Fixation and Permeabilization of Cells for Intracellular Antigen Staining
- 09:25Preparation of Cells for Flow Cytometry and Flow Cytometric Analysis
- 10:34Characterization of Cardiomyocytes by Flow Cytometry and Electrophysiology
- 12:26Conclusion
L'article décrit la méthodologie détaillée pour différencier efficacement les cellules souches pluripotentes humaines dans les cardiomyocytes en modulant de manière sélective la voie Wnt, suivie d'une analyse par cytométrie en flux des marqueurs de référence pour évaluer l'homogénéité et l'identité de la population.
