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High Efficiency Differentiering af humane pluripotente stamceller til hjertemuskelceller og karakterisering ved hjælp af flowcytometri
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High Efficiency Differentiering af humane pluripotente stamceller til hjertemuskelceller og karakterisering ved hjælp af flowcytometri

Article DOI: 10.3791/52010-v • 13:13 min • September 23rd, 2014
September 23rd, 2014 •
*1, *2, 1, 1, 3, 2, 4,5, 1,6
1Department of Biochemistry, Medical College of Wisconsin, 2Stanford Cardiovascular Institute, Stanford University School of Medicine, 3Department of Anesthesiology, Medical College of Wisconsin, 4Stem Cell and Regenerative Medicine Consortium, LKS Faculty of Medicine, Hong Kong University, 5Division of Cardiology, Johns Hopkins University School of Medicine, 6Cardiovascular Research Center, Biotechnology and Bioengineering Center, Medical College of Wisconsin
* These authors contributed equally

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Artiklen beskriver den detaljerede metode til effektivt at differentiere humane pluripotente stamceller til kardiomyocytter ved selektivt at modulere Wnt vej, efterfulgt af flowcytometri analyse af referencemarkører at vurdere homogenitet og identitet af befolkningen.

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Cellebiologi menneskeskabte pluripotente stamceller flowcytometri instrueret differentiering cardiomyocyte IRX4 TNNI3 TNNT2 MCL2v MLC2a
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