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Medicine
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人掌腱膜挛缩的<em>离体</em>文化为肌成纤维细胞的研究和细胞外基质相互作用
JoVE Journal
Medicine
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JoVE Journal
Medicine
Human Dupuytren’s
Ex Vivo
Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions
Please note that all translations are automatically generated.
Click here for the English version.
人掌腱膜挛缩的<em>离体</em>文化为肌成纤维细胞的研究和细胞外基质相互作用
DOI:
10.3791/52534-v
•
08:59 min
•
April 18, 2015
•
Sofia Karkampouna
,
Peter Kloen
,
Miryam C. Obdeijn
,
Scott M. Riester
,
Andre J. van Wijnen
5
,
Marianna Kruithof-de Julio
6
1
Department of Molecular Cell Biology, Cancer Genomics Centre and Centre for Biomedical Genetics
,
Leiden University Medical Center
,
2
Department of Orthopedic Surgery
,
Academic Medical Center
,
3
Department of Plastic, Reconstructive and Hand Surgery
,
Academic Medical Center
,
4
Department of Orthopedic Surgery
,
Mayo Clinic
,
5
Department of Biochemistry and Molecular Biology
,
Mayo Clinic
,
6
Department of Dermatology
,
Leiden University Medical Center
Chapters
00:05
Title
02:06
Tissue Preparation and Ex Vivo Tissue Culture Setup
05:27
Combined Second Harmonic Generation (Collagen) and Two-photon Excited Fluorescence (Elastin) Imaging on Ex Vivo Tissue during Culture
06:39
Results: Effects on 3D Extracellular Matrix Composition upon Tissue Manipulation by Compound Treatments and Viral-mediated Gene Modulation
08:17
Conclusion
Summary
Automatic Translation
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Automatic Translation
迪皮特朗病(DD)是手的手掌的纤维增生性疾病。在这里,我们提出了一个协议,从DD文化切除标本的三维(3D)培养体系。这种短期培养系统允许保存的三维结构和纤维化组织的分子性质。
Tags
Dupuytren’s Contracture
Organ Fibrosis
Ex Vivo Culture
Myofibroblasts
Extracellular Matrix
3D Culture
Proliferation
In Vivo Model
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