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JoVE Journal Biology
Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy
Digital Microfluidics for Automated Proteomic Processing
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Digital Microfluidics for Automated Proteomic Processing
Rapid Analysis and Exploration of Fluorescence Microscopy Images
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Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy

Article DOI: 10.3791/52577-v • 06:51 min • February 1st, 2015
February 1st, 2015 •
1
1LOEWE Excellence Cluster for Integrative Fungal Research (IPF), Senckenberg Research Institute

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Summary
Automatic Translation

A convenient and powerful method for studying autophagy in Penicillium chrysogenum by using starvation pads (mixtures of agarose and tap water in a microscope slide containing a central cavity) is presented here.

Tags

Autophagy Analysis Penicillium Chrysogenum Starvation Pads Fluorescence Microscopy Cellular Quality Control Systems Biologically Relevant Molecules Organelle Dynamics Protease Activity Surplus Organelle Degradation Autophagy Regulation Genetic Factors Environmental Factors Filamentous Fungus
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