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Biology
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成像APP的细胞内运输与光活化GFP
JoVE Journal
Biology
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JoVE Journal
Biology
Imaging the Intracellular Trafficking of APP with Photoactivatable GFP
Please note that all translations are automatically generated.
Click here for the English version.
成像APP的细胞内运输与光活化GFP
DOI:
10.3791/53153-v
•
07:55 min
•
October 17, 2015
•
Joshua H. K. Tam
,
Stephen H. Pasternak
2
1
Department of Physiology and Pharmacology, Robarts Research Institute
,
Western University
,
2
Department of Clinical Neurological Sciences
,
Western University
Chapters
00:05
Title
00:54
Cell Transfection and Microscope Stage Preparation
01:52
Photoactivation of ROIs over the Golgi
03:20
Determining the Downstream Compartment
04:00
Ensuring Accuracy of Photoactivation within the Golgi
05:42
Results: Photoactivatable Intracellular Trafficking Analysis Using βAPP-paGFP Photoactivation
07:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
而细胞表面蛋白的传输相对容易地研究,可视化细胞内蛋白质的贩卖是困难得多。在这里,我们使用结合光活化绿色荧光蛋白的结构和表现出的方法来准确地遵循从高尔基体向下游车厢淀粉样前体蛋白,并按照其清除。
Tags
Amyloid Precursor Protein (APP)
Beta-amyloid (Aβ)
Alzheimer’s Disease
Photoactivatable GFP (paGFP)
Intracellular Trafficking
Golgi
Endosomes
Lysosomes
Secretase Inhibitors
Pulse-chase Experiments
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