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Imaging de intracellulære Trafficking af APP med fotoaktiverbart GFP
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JoVE Journal Biology
Imaging the Intracellular Trafficking of APP with Photoactivatable GFP

Imaging de intracellulære Trafficking af APP med fotoaktiverbart GFP

DOI:
10.3791/53153-v

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07:55 min

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October 17, 2015

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1Department of Physiology and Pharmacology, Robarts Research Institute,Western University, 2Department of Clinical Neurological Sciences,Western University

Chapters

  • 00:05Title
  • 00:54Cell Transfection and Microscope Stage Preparation
  • 01:52Photoactivation of ROIs over the Golgi
  • 03:20Determining the Downstream Compartment
  • 04:00Ensuring Accuracy of Photoactivation within the Golgi
  • 05:42Results: Photoactivatable Intracellular Trafficking Analysis Using βAPP-paGFP Photoactivation
  • 07:21Conclusion

Summary

Automatic Translation

Automatic Translation

Mens transport af celleoverfladeproteiner relativt let studeres, visualisere handel med intracellulære proteiner er meget vanskeligere. Her bruger vi konstruktioner inkorporerer fotoaktiverbare GFP og demonstrere en metode til nøjagtigt at følge amyloidprecursorproteinet fra Golgi-apparatet til ned-stream rum og følge dets clearance.

Tags

Amyloid Precursor Protein (APP)Beta-amyloid (Aβ)Alzheimer’s DiseasePhotoactivatable GFP (paGFP)Intracellular TraffickingGolgiEndosomesLysosomesSecretase InhibitorsPulse-chase Experiments

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