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Gebruikmakend Digital Droplet PCR om BRAF V600E Mutaties Detect in formaline gefixeerde paraffine-ingebedde Reference Standard cellijnen
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Gebruikmakend Digital Droplet PCR om BRAF V600E Mutaties Detect in formaline gefixeerde paraffine-ingebedde Reference Standard cellijnen

Article DOI: 10.3791/53190-v • 10:16 min • October 8th, 2015
October 8th, 2015 •
1, 3, 1, 3, 4, 2, 2, 1,2
1Research Institute of Pharmaceutical Science, Department of Pharmacy, College of Pharmacy, Seoul National University, 2The Center for Anti-Cancer CDx, N-Bio, Seoul National University, 3ABION CRO, 4ABION Inc., R&D Center

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Het doel van deze video wijzen voor geautomatiseerde DNA-extractie van formaline gefixeerde in paraffine ingebedde (FFPE) referentiestandaard cellijnen en digitale druppeltje PCR (ddPCR) analyse zeldzame mutaties te detecteren in een klinische setting voeren. Detecteren van mutaties in FFPE monsters toont de klinische bruikbaarheid van ddPCR in FFPE monsters.

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Molecular Biology DNA-mutatie BRAF ddPCR moleculaire biologie Fluorescentie FFPE Cancer biologie
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