Journal
/
Bioengineering
/
Hul fiber Bioreaktorer for<em> In vivo</em> -lignende Pattedyr Tissue Culture
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture

Hul fiber Bioreaktorer for<em> In vivo</em> -lignende Pattedyr Tissue Culture

DOI:
10.3791/53431-v

•

08:28 min

•

May 26, 2016

•

, , , , , , ,
1Department of Chemical Engineering and Centre for Regenerative Medicine,University of Bath, 2MRC Centre for Drug Safety Science and Institute of Translational Medicine,University of Liverpool, 3Mechanical Engineering,University College London, 4Department of Applied Mathematics,Liverpool John Moores University

Chapters

  • 00:05Title
  • 00:35Module Fabrication
  • 01:58System Setup and Sterilization
  • 04:12Seeding the Module
  • 06:01Excision of Fibers
  • 06:44Results: Seeding, Proliferation, Viability and Biochemical Analysis
  • 08:06Conclusion

Summary

Automatic Translation

Automatic Translation

Funktionelle respons af celler i kultur kan forbedres ved dyrkning i mere in vivo-lignende 3-dimensionelle kultur miljøer 16-21. Dette håndskrift beskriver opsætning og drift af en hul fiber bioreaktor-system til in vivo-lignende pattedyr vævskultur.

Tags

Hollow Fiber BioreactorIn Vitro ModelLiver Cell FunctionPhysiologically Relevant EnvironmentSinusoidBile CanaliculiCell CultureDrug DosingBorosilicate GlassSiliconizingAutoclaveSilicone GluePTFE TapeEthanol SterilizationPump TubingModule ConnectorsPermeate LineRetentate Line

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Hollow mikro-baserede sensor for Multiplexed Transdermal Elektrokemisk Sensing

Selvrapportering Stilladser for 3-Dimensional Cell Culture

Generation af en tre-dimensionel fuld tykkelse Skin Equivalent og Automated Anskydning

Dyrkning Mus hjerteklapper i Miniature Tissue Culture System

Sandwich-lignende mikromiljøer at udnytte Cell / Materiale Interactions

En Mikrofluid platform for High-throughput Single-celle Isolering og Kultur

Multimodal Imaging og spektroskopi Fiber-bundle Microendoscopy platform for ikke-invasiv,<em> In vivo</em> Analyse Tissue

Power Input målinger i Stirred bioreaktorer på laboratoriet skala

Custom manipuleret vævskultur forme fra Laser-ætset Masters

Read Article