Technique to Target Microinjection to the Developing Xenopus Kidney

Bridget D. DeLay; Vanja Krneta-Stankic; Rachel K. Miller

doi:10.3791/53799

Journal

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Developmental Biology

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Методика Целевой показатель микроинъекции к проявляющему Xenopus почки

Technique to Target Microinjection to the Developing <em>Xenopus</em> Kidney

JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology

Technique to Target Microinjection to the Developing Xenopus Kidney

Методика Целевой показатель микроинъекции к проявляющему Xenopus почки

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11,172 Views

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11:29 min

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May 03, 2016

DOI:

10.3791/53799-v

DOI:

10.3791/53799-v

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11:29 min

May 03, 2016

•

1 Views

Bridget D. DeLay¹, Vanja Krneta-Stankic^1,2, Rachel K. Miller^1,2,3,4

¹Department of Pediatrics, Pediatric Research Center,University of Texas McGovern Medical School, ²Program in Genes & Development,University of Texas Graduate School of Biomedical Sciences, ³Program in Cell & Regulatory Biology,University of Texas Graduate School of Biomedical Sciences, ⁴Department of Genetics,University of Texas MD Anderson Cancer Center

Transcript

Summary

Here, we present a protocol to use fate maps and lineage tracers to target injections into individual blastomeres that give rise to the kidney of Xenopus laevis embryos.

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Microinjection Xenopus Kidney Developmental Biology Testis Isolation Egg Collection Fertilization De-jelling Temperature Control Membrane-bound Red Fluorescent Protein MRNA

Методика Целевой показатель микроинъекции к проявляющему<em> Xenopus</em> почки

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