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自发性乳腺癌转移的原位小鼠模型
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JoVE Journal Medicine
An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis
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自发性乳腺癌转移的原位小鼠模型

DOI:
10.3791/54040-v

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08:11 min

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August 14, 2016

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1Department of Biochemistry and Molecular Biology, Medical College of Georgia,Augusta University, 2Georgia Cancer Center,Augusta University, 3Charlie Norwood VA Medical Center

Chapters

  • 00:05Title
  • 00:48Orthotopic Breast Cancer Tumor Establishment
  • 02:17Live Mouse Bioluminescent Tumor Growth Imaging
  • 03:24Surgical Primary Tumor Removal
  • 04:36Lung Metastases Validation
  • 06:13Results: Representative Evaluation of Spontaneous Breast Cancer Metastasis
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

原位乳腺癌原发肿瘤模型和手术切除原发肿瘤，延长生命鼠标产生自发转移的描述。肿瘤的生长和发展进行监测和通过萤光素酶荧光成像定量。

Tags

Orthotopic Mouse ModelSpontaneous Breast Cancer Metastasis4T1 Luciferase CellsBALB/c MiceBioluminescence ImagingPrimary Tumor GrowthMetastasis Kinetics

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