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Journal / Developmental Biology / 쥐 배아 Hemogenic 내피 세포의 분리
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Developmental Biology

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JoVE Journal Developmental Biology
쥐 배아 Hemogenic 내피 세포의 분리
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쥐 배아 Hemogenic 내피 세포의 분리

Article DOI: 10.3791/54150-v • 08:56 min • June 17th, 2016
June 17th, 2016 •
*1, *2, 3, 1
1Departments of Medicine, Genetics and Biomedical Engineering, Yale Cardiovascular Research Center, Vascular Biology and Therapeutics Program, Yale Stem Cell Center, Yale University School of Medicine, 2Department of Pediatrics, Section of Neonatal-Perinatal Medicine, Yale University School of Medicine, 3Department of Molecular and Cellular Biology, Baylor College of Medicine
* These authors contributed equally

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조혈 줄기 및 전구 세포 (HSPC)는 아직 작은 일부 내피 세포는 혈액이 형성되기 위해 지정하는 과정에 대해 알려진, 개발 과정에서 전문 (hemogenic) 내피 세포에서 파생. 우리는 뮤린 배아 조직으로부터 hemogenic 내피 세포 HSPC 동시에 아이솔레이션을 허용하는 유동 세포 계측법 기반 방법을 보여준다.

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발달 생물학 문제 (112) hemogenic 내피 세포 최종 조혈 혈관 개발 마우스 배아 FACS 조혈 줄기 및 전구 세포
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