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Journal / Developmental Biology / 쥐 심장의 심실 무료 벽에서 내심 내피 및 관상 내피 세포의…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
쥐 심장의 심실 무료 벽에서 내심 내피 및 관상 내피 세포의 격리
Generation of a Human iPSC-Based Blood-Brain Barrier Chip
Journal (Developmental Biology)
Generation of a Human iPSC-Based Blood-Brain Barrier Chip
The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
Journal (Developmental Biology)
The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the Single-cell Level: Labeling by Antibody Staining, RNAi Loss-of-function Analysis and Imaging
 
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쥐 심장의 심실 무료 벽에서 내심 내피 및 관상 내피 세포의 격리

Article DOI: 10.3791/61126-v • 08:22 min • April 15th, 2020
April 15th, 2020 •
1,2,3, 1,2,3, 1,2,3,4,5, 1,2,3,4,5
1Department of Pediatrics, Division of Cardiology, Stanford School of Medicine, 2Vera Moulton Wall Center for Pulmonary Vascular Disease, Stanford School of Medicine, 3Stanford Cardiovascular Institute, Stanford School of Medicine, 4Division of Pulmonary Biology, Department of Pediatrics, Cincinnati Children's Hospital Medical Center, 5Center for Stem Cell and Organoid Medicine, CuSTOM, Division of Developmental Biology, and Perinatal Institute, Cincinnati Children's Hospital Medical Center

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우리는 소화 완충제에서 순차적 조직 소화를 통해 쥐 심혼에서 내심 과 관상 관상 내피 세포의 격리를 위한 프로토콜을 제시하고, 재발하는 원심분리기 주기에서 세포 수집 및 반대로 쥐 CD31 마이크로비드를 사용하여 세포 정화.

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발달 생물학 문제 158 심내막 관상 동맥 내피 격리 심장 쥐 CD31
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