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单队列殖民地和工蜂的激素治疗的准备来分析生理学与关联作用和/或内分泌系统

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Neuroscience

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JoVE Journal Neuroscience

Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

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单队列殖民地和工蜂的激素治疗的准备来分析生理学与关联作用和/或内分泌系统

DOI:

10.3791/54240-v

•

08:53 min

•

September 06, 2016

•

Takayuki Ueno², Kiyoshi Kawasaki, Takeo Kubo

¹Department of Biological Sciences, Graduate School of Sciences,The University of Tokyo, ²Faculty of Pharmaceutical Sciences,Doshisha Women’s College

Chapters

00:05Title
00:36Preparation of Single-cohort Colonies
02:28Classifying Hypopharyngeal Glands (HPGs)
04:09Injection of Active Ecdysone (20E)
05:24Application of Methoprene, a JH Analogue
06:23Results: mrjp2 and Hbg3 Expression in the HPG of Worker Honeybees
07:48Conclusion

Summary

Automatic Translation

在这里，我们描述详细的协议单队列蜂群的准备 - 分析相关角色工人生理的有用工具。我们还描述了用于治疗工人幼激素和蜕皮激素评估这些激素在工作者行为和/或生理调控的参与详细协议。

Tags

Single-cohort Colony Worker Honeybees Physiology Role Endocrine System Worker Behavior Newly-emerged Workers Nurse Bees Precocious Foragers Hypopharyngeal Glands (HPGs)

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