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Analysis of Fatty Acid Content and Composition in Microalgae

Analysis of Fatty Acid Content and Composition in Microalgae

Article DOI: 10.3791/50628 07:44 min October 1st, 2013
October 1st, 2013



A method for the determination of fatty acid content and composition in microalgae based on mechanical cell disruption, solvent based lipid extraction, transesterification, and quantification and identification of fatty acids using gas chromatography is described. A tripentadecanoin internal standard is used to compensate for the possible losses during extraction and incomplete transesterification.


Fatty Acid Content Fatty Acid Composition Microalgae Acyl-lipid Classes Triacylglycerol (TAG) Commercial Interest Transportation Fuels Bulk Chemicals Nutraceuticals Food Commodities Analytical Methods Quantification Cell Disruption Lipid Extraction Transesterification Fatty Acid Methyl Esters (FAMEs)
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