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CUBIC协议可视化在全山皮肤配方单细胞分辨率蛋白表达
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JoVE Journal Biology
CUBIC Protocol Visualizes Protein Expression at Single Cell Resolution in Whole Mount Skin Preparations
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CUBIC协议可视化在全山皮肤配方单细胞分辨率蛋白表达

DOI:
10.3791/54401-v

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08:09 min

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August 04, 2016

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, , , , ,
1The School of Medical Sciences,University of New South Wales Australia, 2Neuroscience Research Australia, 3Biomedical Imaging Facility,University of New South Wales Australia

Chapters

  • 00:05Title
  • 01:19Transparent Mouse Skin Tissue Preparation
  • 02:32Mouse Skin Biopsy Clearance
  • 04:27Mouse Skin Biopsy Imaging
  • 05:08Results: Single Cell Resolution Protein Expression in Whole Mount Skin Preparations
  • 06:55Conclusion

Summary

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这份报告描述了三次协议，以澄清全层小鼠皮肤活检，并在3D单细胞分辨率的可视化表达方式，增殖细胞，皮脂腺细胞和。这种方法使皮肤的解剖学和病理学的准确评估，并在转基因小鼠品系的表皮异常表型。

Tags

CUBIC ProtocolProtein ExpressionSingle Cell ResolutionWhole Mount Skin PreparationsEpidermal DevelopmentSkin RegenerationSkin DiseaseHair FolliclesSkin BiopsySkin ClearingCUBIC Clearing Solution

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