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高解析度全山<em>在原位</em杂交斑马鱼胚胎内，研究基因表达和功能

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Biology

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JoVE Journal Biology

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

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高解析度全山<em>在原位</em杂交斑马鱼胚胎内，研究基因表达和功能

DOI:

10.3791/50644-v

•

10:06 min

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October 19, 2013

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Babykumari P. Chitramuthu², Hugh P. J. Bennett²

¹Endocrine Research Laboratory and Department of Medicine,Royal Victoria Hospital, ²McGill University Health Centre Research Institute

Chapters

00:05Title
01:31Synthesis of Digoxygenin-labeled RNA Probes
03:32Whole Mount in situ Hybridization
06:55Representative in situ Hybridization Results
09:39Conclusion

Summary

Automatic Translation

在脊椎动物的发育和疾病的基因功能研究的模型，小热带鱼，斑马鱼已成为一种流行。靶基因表达的时间和空间可以由<em在原位</em>杂交。我们改进后的协议允许低非特异性背景信号检测低丰度的成绩单。

Tags

Gene Expression Whole Mount In Situ Hybridization WISH Zebrafish Embryos Antisense RNA Probes Digoxigenin In Vitro Transcription Chromogenic Substrate Alkaline Phosphatase Developmental Stage

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