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Biology

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Imaging G-protein-koblet receptor-medieret Kemotaksi og dens signaleringshændelser i Neutrofil-lignende HL60-celler
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Imaging G-protein-koblet receptor-medieret Kemotaksi og dens signaleringshændelser i Neutrofil-lignende HL60-celler

Article DOI: 10.3791/54511-v • 08:24 min • September 14th, 2016
September 14th, 2016 •
1, 1, 1
1Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health

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Visuelle kemotaksiassays er afgørende for en bedre forståelse af, hvordan eukaryote celler styrer kemoattraktant-medieret retningsbestemt cellevandring. Her beskriver vi detaljerede metoder til: 1) realtid, høj opløsning overvågning af flere kemotaksiassays, og 2) samtidig visualisering af kemoattraktant gradient og spatiotemporale dynamik signalering begivenheder i neutrofil-lignende HL60 celler.

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Cellular Biology Neutrofil kemotaksi G-protein-koblede receptorer (GPCR) G-proteiner signaltransduktion cellemigration
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