Journal
/
Developmental Biology
/
通过注射心脏毒素急性骨骼肌再生的诱导
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection
Please note that all translations are automatically generated. Click here for the English version.

通过注射心脏毒素急性骨骼肌再生的诱导

DOI:
10.3791/54515-v

•

07:39 min

•

January 01, 2017

•

, , , , ,
1Stem Cell Fate Laboratory,Institute of Genetics and Biophysics “A. Buzzati Traverso”, CNR, 2School of Medicine and Surgery,University of Milano-Bicocca, 3Division of Immunology, Transplantation and Infectious Diseases,San Raffaele Scientific Institute

Chapters

  • 00:05Title
  • 00:35Cardiotoxin Injection in the Tibialis Anterior Muscle
  • 01:29Tibialis Anterior Isolation
  • 02:51Fresh Frozen Muscle Technique
  • 04:03Cryostat Sectioning of Frozen Muscles
  • 04:46Hematoxylin & Eosin Stains
  • 05:57Results: Time Course Analysis of Skeletal Muscle Regeneration
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

该原稿描述了详细的协议来诱导成年小鼠和肌肉的随后的操作，如解剖，冷冻，切割，常规染色，和肌纤维横截面积分析急性骨骼肌再生。

Tags

Artificial IntelligenceAI Writing AssistantsAI-generated ContentCopywritingContent GenerationText ProductionAI-powered WritingAutomated WritingAI-assisted Copywriting

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

肾再生的成年斑马鱼庆大霉素性损伤

从人类骨骼肌成肌原细胞和成纤维细胞的分离和定量免疫细胞化学表征

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

通过子代检测改变斑马鱼致死性骨骼突变显

骨骼肌损伤诱导衰老和体内重编程的评价

慢性刺激在收缩活动性大鼠骨骼肌表型适应性研究中的应用

Pax7 和层粘连蛋白抗体免疫荧光法鉴定骨骼肌卫星细胞

完整毛里丝骨骼肌的X射线衍射作为研究肌肉疾病结构基础的工具

从骨骼肌活检程序分离、培养、表征和分化人类肌肉祖细胞

在免疫缺陷小鼠中表演人类骨骼肌异种移植物

Read Article