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Optagelse Gamma Band Svingninger i Pedunculopontine Nucleus neuroner
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Neuroscience
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JoVE Journal Neuroscience
Recording Gamma Band Oscillations in Pedunculopontine Nucleus Neurons

Optagelse Gamma Band Svingninger i Pedunculopontine Nucleus neuroner

DOI:
10.3791/54685-v

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09:04 min

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September 14, 2016

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, , , ,
1IFIBYNE-CONICET,University of Buenos Aires, 2Center for Translational Neuroscience,University of Arkansas for Medical Sciences

Chapters

  • 00:05Title
  • 00:54Solutions Preparation
  • 02:07Slice Preparation
  • 03:12Whole-Cell Patch Clamp Recordings
  • 06:32Results: Gamma Band Membrane Oscillations in Whole-Cell Recorded PPN Neurons Using Current Ramps of Augmenting Amplitude
  • 07:44Conclusion

Summary

Automatic Translation

Automatic Translation

Den pedunculopontine kerne (PPN) er placeret i hjernestammen og dens neuroner maksimalt aktiveret under vågne og hurtige øjenbevægelser (REM) søvn hjernen stater. Dette arbejde beskriver den eksperimenterende tilgang til at optage in vitro gamma band subthreshold membran svingning i PPN neuroner.

Tags

Gamma Band OscillationsPedunculopontine Nucleus NeuronsIntrinsic Membrane PropertiesWhole-cell Patch Clamp RecordingACSF PreparationSucrose ACSFVibratome SlicingBrain Dissection

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