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Biology

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Analyse af SCAP
 
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Analyse af SCAP

Article doi: 10.3791/54709
November 8th, 2016

Summary November 8th, 2016

Please note that all translations are automatically generated.

Click here for the English version.

Vi beskriver en modificeret fremgangsmåde til membranfraktion isoleret fra humane celler og prøveforberedelse til påvisning af SCAP N -glycosylation og totalt protein ved hjælp af Western blot. Vi yderligere indføre en metode GFP-mærkning for at overvåge SCAP menneskehandel hjælp konfokal mikroskopi. Denne protokol kan bruges i almindelige biologiske laboratorier.

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