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Biochemistry
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SorLA和CLC:这表现在免疫印迹,溶酶体酶的抑制,和免疫组化CNTFRα的CLF-1依赖性下调
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
Please note that all translations are automatically generated.
Click here for the English version.
SorLA和CLC:这表现在免疫印迹,溶酶体酶的抑制,和免疫组化CNTFRα的CLF-1依赖性下调
DOI:
10.3791/55019-v
•
10:16 min
•
January 06, 2017
•
Jakob V. Larsen
,
Claus M. Petersen
1
Department of Biomedicine
,
Aarhus University
Chapters
00:05
Title
00:55
Western Blotting of CNTFRα Lysates
03:21
Immunocytochemistry in Combination with Lysosomal Inhibitors
06:00
Western Blot-detection of the pSTAT3 Levels
08:17
Results: Demonstration of CNTFRα-downregulation by Western Blotting, and Inhibition of Lysosomal Enzymes, and Immunocytochemistry
09:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述印迹和免疫细胞化学以抑制溶酶体酶的组合如何西方可以用来演示睫状神经营养因子受体α(CNTFRα),它是由细胞因子样因子1(CLF-1之间的相互作用输送的下调)和内吞受体sorLA。
Tags
SorLA
CLC:CLF-1
CNTFR
Western Blotting
Immunocytochemistry
Lysosomal Degradation
GPI-anchored Receptor
Protein Turnover
Stable Cell Lines
Transfectants
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