Journal
/
/
SorLA og CLC: CLF-1-afhængig Nedregulering af CNTFRa som demonstreret ved Western blotting, Inhibering af lysosomale enzymer, og Immunocytokemi
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
DOI:

10:16 min

January 06, 2017

,

Chapters

  • 00:05Title
  • 00:55Western Blotting of CNTFRα Lysates
  • 03:21Immunocytochemistry in Combination with Lysosomal Inhibitors
  • 06:00Western Blot-detection of the pSTAT3 Levels
  • 08:17Results: Demonstration of CNTFRα-downregulation by Western Blotting, and Inhibition of Lysosomal Enzymes, and Immunocytochemistry
  • 09:43Conclusion

Summary

Automatic Translation

Den nuværende protokol beskriver, hvordan Western blotting og immuncytokemi kombineret med hæmning af lysosomale enzymer kan bruges til at demonstrere nedregulering af ciliarneurotrofiske Factor receptor-α (CNTFRa), der transporteres af samspillet mellem Cytokine-lignende faktor-1 (CLF-1 ) og den endocytiske receptor sorLA.

Related Videos

Read Article