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SorLA og CLC: CLF-1-afhængig Nedregulering af CNTFRa som demonstreret ved Western blotting, Inhibering af lysosomale enzymer, og Immunocytokemi
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Chapters
- 00:05Title
- 00:55Western Blotting of CNTFRα Lysates
- 03:21Immunocytochemistry in Combination with Lysosomal Inhibitors
- 06:00Western Blot-detection of the pSTAT3 Levels
- 08:17Results: Demonstration of CNTFRα-downregulation by Western Blotting, and Inhibition of Lysosomal Enzymes, and Immunocytochemistry
- 09:43Conclusion
Den nuværende protokol beskriver, hvordan Western blotting og immuncytokemi kombineret med hæmning af lysosomale enzymer kan bruges til at demonstrere nedregulering af ciliarneurotrofiske Factor receptor-α (CNTFRa), der transporteres af samspillet mellem Cytokine-lignende faktor-1 (CLF-1 ) og den endocytiske receptor sorLA.
