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JoVE Journal Immunology and Infection
Genoom-brede analyse van HDAC-Inhibitor-gemedieerde modulatie van microRNAs en mRNAs in B cellen geïnduceerde Undergo klasse-switch DNA recombinatie en Plasma celdifferentiatie
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Genoom-brede analyse van HDAC-Inhibitor-gemedieerde modulatie van microRNAs en mRNAs in B cellen geïnduceerde Undergo klasse-switch DNA recombinatie en Plasma celdifferentiatie

Article DOI: 10.3791/55135-v • 11:06 min • September 20th, 2017
September 20th, 2017 •
1, 1,2, 1,3, 1,3, 1, 1
1Department of Microbiology, Immunology and Molecular Genetics, University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine, Cental South University, 3Greehey Children’s Cancer Research Institute, University of Texas Long School of Medicine at San Antonio

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Epigenetische factoren kunnen communiceren met genetische programma's voor het moduleren van genexpressie en de regulering van de B-cel functie. Door stimulatie van de in vitro B-cel, qRT-PCR, en high-throughput microRNA-sequence en mRNA-sequence benaderingen te combineren, kunnen we analyseren de epigenetische modulatie van miRNA en gen-expressie in B-cellen.

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Immunologie kwestie 127 B-cel klasse-switch DNA recombinatie stroom cytometry histone deacetylase (HDAC) remmers (Savoie) mRNA microRNA mRNA-seq miRNA-seq plasma celdifferentiatie
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