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Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants
JoVE Journal
Environment
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JoVE Journal Environment
Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants

Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants

DOI:
10.3791/55211-v

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11:26 min

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May 26, 2017

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, , , , ,
1Institute for Environmental Protection and Research (ISPRA), Rome (Italy), 2Department of Biology,Tor Vergata University, Rome (Italy), 3Department of Biology and Evolution of Marine Organisms,Stazione Zoologica, Anton Dohrn, Naples (Italy)

Chapters

  • 00:00Title
  • 00:06Introduction
  • 00:53Preparation of Reagents and Materials
  • 04:16Bacterial Inoculum Preparation
  • 06:20Testing Exposure
  • 08:21Count Colony Forming Units (CFU)
  • 09:13Representative Results
  • 09:53Conclusion

Summary

Automatic Translation

Automatic Translation

This work describes a new protocol to assess the ecotoxicity of pollutants, including emerging contaminants such as nanomaterials, using the marine bacterium Vibrio anguillarum. This method allows for the determination of the LC50, or mortality, the concentration that causes a 50% decrease in bacteria culturability, after a 6 h exposure.

Tags

Keywords: Ecotoxicological MethodMarine BacteriaVibrio AnguillarumAcute ToxicityEnvironmental ContaminantsBioindicatorsToxicity TestingSerial DilutionPetri DishesTryptic Soy AgarTryptic Soy BrothSodium ChlorideCopper SulfateReference ToxicantEnvironmental Samples

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