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Immunology and Infection

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JoVE Journal Immunology and Infection
Intracellulaire kleuring en flowcytometrie om lymfocyten in muizen Aorta, nieren en lymfeklieren te identificeren in een Model of Hypertension
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Intracellulaire kleuring en flowcytometrie om lymfocyten in muizen Aorta, nieren en lymfeklieren te identificeren in een Model of Hypertension

Article DOI: 10.3791/55266-v • 09:20 min • January 28th, 2017
January 28th, 2017 •
1, 2, 3, 1
1Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University

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Dit artikel bevat gedetailleerde methodologie voor het identificeren en kwantificeren van functionele T-lymfocyten subsets in muizen nieren, aorta en de lymfeklieren aanwezig door intracellulaire kleuring en flowcytometrie. Het model van angiotensine II geïnduceerde hypertensie werd gekozen uitleggen, stap voor stap, de procedures en de grondbeginselen van flowcytometrie en intracellulaire kleuring.

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Immunologie intracellulaire kleuring flowcytometrie T-cellen lymfocyten cytokinen hypertensie
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