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Evaluación de ubicación intracelular de especies reactivas de oxígeno en espermatozoides de Solea Senegalensis

Article DOI: 10.3791/55323-v • 10:24 min • March 11th, 2018
March 11th, 2018 •
1,2, 2,3
1Department of Molecular Biology, University of León, 2INDEGSAL, University of León, 3Planta de Cultivos el Bocal, Spanish Institute of Oceanography (IEO)

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Este protocolo describe una metodología detallada para detectar la localización de2 H2O en Solea senegalensis espermatozoides con un fluorocromo sensible DCFH-DA para ROS, una mancha de mitocondrias vivo para las mitocondrias y DAPI para núcleos visualización, respectivamente. El protocolo está diseñado para ser realizado dentro de 2 h con espermatozoides frescos o descongelados.

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Biología del desarrollo cuestión 133 Solea Senegalensis espermatozoides mitocondrias criopreservación las mitocondrias de la mancha DCFH-DA DAPI Microscopía Confocal
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