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Journal / Developmental Biology / Evaluering af intracellulær placering af reaktive …
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Developmental Biology
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JoVE Journal Developmental Biology
Evaluering af intracellulær placering af reaktive ilt arter i Solea Senegalensis sædcellerne
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Evaluering af intracellulær placering af reaktive ilt arter i Solea Senegalensis sædcellerne

Article DOI: 10.3791/55323-v • 10:24 min • March 11th, 2018
March 11th, 2018 •
1,2, 2,3
1Department of Molecular Biology, University of León, 2INDEGSAL, University of León, 3Planta de Cultivos el Bocal, Spanish Institute of Oceanography (IEO)

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Denne protokol beskriver en detaljeret metode til påvisning af H2O2 lokalisering inden for Solea senegalensis sædcellerne ved hjælp af en følsom fluorokrom DCFH-DA for ROS, en levende mitokondrier pletten for mitokondrier og DAPI for kerner visualisering, henholdsvis. Protokollen er designet til at blive udført inden for 2 h med enten friske eller optøede sædcellerne.

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Udviklingsmæssige biologi sag 133 Solea Senegalensis sædcellerne mitokondrier kryopræservering mitokondrier pletten DCFH-DA DAPI konfokalmikroskopi
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