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Journal / Immunology and Infection / Live avbildning antimykotiskt av humana primära…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Live avbildning antimykotiskt av humana primära neutrofiler och monocyter som svar på
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Live avbildning antimykotiskt av humana primära neutrofiler och monocyter som svar på

Article DOI: 10.3791/55444-v • 12:29 min • April 19th, 2017
April 19th, 2017 •
1, 1, 1, 2, 2, 2, 1
1Aberdeen Fungal Group, MRC Centre for Medical Mycology, Institute of Medical Sciences, University of Aberdeen, 2Department of Medicine, Memorial Sloan-Kettering Cancer Center, New York, US

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Här beskriver vi ett protokoll för att bedöma antisvampaktivitet av primära humana immunceller i realtid med hjälp av fluorescerande Aspergillus reporter konidier i samband med live-cell video mikroskopi och flödescytometri. Genererade data ge insikt i värd cell- Aspergillus interaktioner såsom fungicid aktivitet, fagocytos, cellmigration och inhibition av svamptillväxt.

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Immunology , Levande cell imaging antifungal aktivitet signalljus humana leukocyter värd-patogen-interaktion.
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