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Developmental Biology

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JoVE Journal Developmental Biology
Eine einfache Methode zur Identifizierung von Kinasen, die die embryonale Stammzell-Pluripotenz durch Hochdurchsatz-Inhibitor-Screening regulieren
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Eine einfache Methode zur Identifizierung von Kinasen, die die embryonale Stammzell-Pluripotenz durch Hochdurchsatz-Inhibitor-Screening regulieren

Article DOI: 10.3791/55515-v • 07:18 min • May 12th, 2017
May 12th, 2017 •
1, 2,3, 1
1The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2Department of Cancer Biology, Dana-Farber Cancer Institute, 3Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

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Hier stellen wir ein quantitatives und skalierbares Protokoll vor, um gezielte kleine Molekülbildschirme für Kinase-Regulatoren des naiv-grundierten pluripotenten Übergangs durchzuführen.

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Entwicklungsbiologie Ausgabe 123 Proteinkinase Embryonale Stammzelle Pluripotenz Naive-Primed-Übergang Kinase-Inhibitoren Hochdurchsatz-Screen
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