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Journal / Developmental Biology / Lysmediert reversibel modulering av den…
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JoVE Journal Developmental Biology
Lysmediert reversibel modulering av den mitogenaktiverte proteinkinaseveien under celledifferensiering og
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Lysmediert reversibel modulering av den mitogenaktiverte proteinkinaseveien under celledifferensiering og

Article DOI: 10.3791/55823-v • 09:32 min • June 15th, 2017
June 15th, 2017 •
*1, *2, 1, 1, 1, 2, 2, 1,3,4
1Department of Biochemistry, University of Illinois at Urbana-Champaign, 2Department of Comparative Biosciences, University of Illinois at Urbana-Champaign, 3Neuroscience Program, University of Illinois at Urbana-Champaign, 4Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign
* These authors contributed equally

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Denne protokollen beskriver en optogenetisk strategi for å modulere mitogenaktivert proteinkinase (MAPK) aktivitet under celledifferensiering og utvikling av Xenopus embryon. Denne metoden tillater reversibel aktivering av MAPK-signalveien i pattedyrcellekultur og i multicellulære levende organismer, som Xenopus- embryoer, med høy romlig og tidsmessig oppløsning.

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Utviklingsbiologi utgave 124 optogenetikk protein-protein-interaksjoner PC12-celledifferensiering, Embryonisk utvikling kryptokrom CRY2-CIBN bicistronisk Raf / MEK / ERK kinase signaleringskaskade
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