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In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana

Article DOI: 10.3791/55975-v • 05:41 min • September 11th, 2017
September 11th, 2017 •
*1,2, *1, 1,2,3, 1,3
1Division of Biological Science, Graduate School of Science, Nagoya University, 2Higashiyama Live-Holonics Project, JST-ERATO, Nagoya University, 3Institute of Transformative Bio-Molecules (ITbM), Nagoya University
* These authors contributed equally

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Summary
Automatic Translation

This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.

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In Vitro Ovule Cultivation Live-cell Imaging Zygote Polarization Embryo Patterning Arabidopsis Thaliana Plant Development Embryogenesis Dynamics Micropillar Device PDMS Micropillar Array N5T Medium Vacuum Chamber Degassing
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