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JoVE Journal
Biochemistry

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从交联骨骼肌制备高质量核的基于过滤的方法进行染色质免疫沉淀
 
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从交联骨骼肌制备高质量核的基于过滤的方法进行染色质免疫沉淀

Article DOI: 10.3791/56013-v 10:04 min July 6th, 2017
July 6th, 2017

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

我们提出了基于过滤的方案,以将高质量的细胞核与交联的小鼠骨骼肌分离,其中我们消除了超离心的需要,使其易于应用。我们显示从核制备的染色质适用于染色质免疫沉淀和可能的染色质免疫沉淀测序研究。

Tags

生物化学,第125期,骨骼肌,交联,核分离,染色质免疫沉淀,顺序过滤,昼夜节律
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