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Journal / Neuroscience / Live Imaging af primære hjernebarken celler ved…
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Neuroscience

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JoVE Journal Neuroscience
Live Imaging af primære hjernebarken celler ved hjælp af en 2D kultur
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Live Imaging af primære hjernebarken celler ved hjælp af en 2D kultur

Article DOI: 10.3791/56063-v • 10:12 min • August 9th, 2017
August 9th, 2017 •
1, 1, 2, 3, 1
1Brain Institute, Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering, ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University

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Live imaging er et kraftfuldt værktøj til at studere cellulær adfærd i realtid. Her, beskriver vi en protokol for time-lapse video-mikroskopi af primære hjernebarken celler, der giver mulighed for en detaljeret gennemgang af de faser, der er vedtaget under lineage progression fra primære neurale stamceller til differentieret neuroner og glia.

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Neurobiologi sag 126 primære cellekultur hjernebarken time-lapse video-mikroskopi celleproliferation neuronal differentiering celle overlevelse.
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