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November 08, 2017
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Este protocolo se describe en detalle la generación de células libres de huella pluripotentes inducidas (iPSCs) de las células pancreáticas humanas en condiciones libres de alimentador, seguido de edición utilizando ribonucleoproteínas CRISPR/Cas9 y caracterización de la modificación sola célula clones.
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Cite this Article
Nandal, A., Mallon, B., Telugu, B. P. Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System. J. Vis. Exp. (129), e56260, doi:10.3791/56260 (2017).
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