Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Effektiv Generation og redigering af…
JoVE Journal
Bioengineering

A subscription to JoVE is required to view this content.

JoVE Journal Bioengineering
Effektiv Generation og redigering af Feeder-gratis IPSCs fra pancreas celler ved hjælp af CRISPR-Cas9 System
Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
Journal (Bioengineering)
Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization
Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
Journal (Bioengineering)
Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
 
Click here for the English version

Effektiv Generation og redigering af Feeder-gratis IPSCs fra pancreas celler ved hjælp af CRISPR-Cas9 System

Article DOI: 10.3791/56260 • 09:16 min • November 8th, 2017
November 8th, 2017 •
1,2, 3, 1,2,4
1Department of Animal and Avian Sciences, University of Maryland, 2Animal Bioscience and Biotechnology Laboratory, ARS, USDA, 3NIH Stem Cell Unit, Bethesda, National Institutes of Health, 4RenOVAte Biosciences Inc

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne protokol beskriver i detaljer generation af fodaftryk-fri induceret pluripotente stamceller (iPSCs) fra pancreas menneskeceller i feeder-fri betingelser, efterfulgt af redigering bruger CRISPR/Cas9 ribonucleoproteins og karakterisering af den modificerede encellede kloner.

Tags

Bioteknologi sag 129 Induced pluripotente stamceller (iPSCs) omprogrammering sendai virus CRISPR/Cas9 fodaftryk-fri iPSCs nucleofection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter