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Journal / Immunology and Infection / Inriktning cystein tioler för in Vitro…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Inriktning cystein tioler för in Vitro platsspecifika Glycosylation av rekombinanta proteiner
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Inriktning cystein tioler för in Vitro platsspecifika Glycosylation av rekombinanta proteiner

Article DOI: 10.3791/56302-v • 11:25 min • October 4th, 2017
October 4th, 2017 •
*1, *1, *1, *1, 1, 1
1Department of Physiology and Pharmacology, Schulich School of Medicine and Dentistry, University of Western Ontario
* These authors contributed equally

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Biokemiska och strukturella analyser av glykosylerat proteiner kräver relativt stora mängder homogena prover. Här presenterar vi en effektiv kemisk metod för platsspecifika glycosylation av rekombinanta proteiner renas från bakterier genom att rikta reaktiva Cys tioler.

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Immunologi fråga 128 glycosylation methanethiosulfonate cystein thiol modifiering stromal interaktion molekyl-1 EFSAM lösning kärnmagnetisk resonansspektroskopi masspektrometri
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